Bacteriostatic Water is sterile water containing a small amount of benzyl alcohol, designed to inhibit bacterial growth after opening.

In laboratory settings, it is commonly used for the reconstitution of lyophilized peptides and research compounds, allowing for accurate dilution and preparation.

Required Materials

• Peptide vial (3 ml)
• Bacteriostatic water (3 mL)
• 3 mL syringe
• 27G x 16 mm needle (or equivalent)
• Alcohol swabs

Procedure

1. Prepare a sterile workspace

• Wash your hands thoroughly prior to handling any materials.
• Ensure your workspace is clean and free from contaminants.
• Using an alcohol swab, disinfect the rubber stoppers of both the peptide vial and the bacteriostatic water vial.

2. Draw up bacteriostatic water

• Using a 3ml syringe with an appropriate needle, withdraw 3ml of bacteriostatic water.
• Ensure accurate measurement and avoid introducing air bubbles where possible.

3. Reconstitute the peptide

• Insert the needle into the peptide vial and slowly inject the bacteriostatic water.
• Direct the stream of liquid down the inner wall of the vial to minimise agitation of the lyophilised powder.

4. Allow the peptide to dissolve

• Allow the vial to stand for approximately 4–5 minutes to enable full dissolution.
• If required, gently swirl the vial to assist mixing.
• Do not shake, as this may compromise peptide integrity.